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Issues with residues and structure changing with FlexPepDock; is this normal?

Category: 
Docking

This is probably a silly question so I apologize if so, but recently lab has been investigating some novel interaction sites between a protein and peptide based off of recent crosslinking and IP data. I created some pdb files of the protein and the peptide aligned at the various novel and established crosslinking sites and submitted them to FlexPepDock to see if the models were reasonable.

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Problem with the top 25 fragments used in the library of 200 nine-residue fragments in AbinitioRelax

Category: 
Structure prediction

Hi,all.

I'm confused about the reason for using the top  25 fragments in the library of 200 nine-residue fragments in AbinitioRelax.Instead,for the library of 200 three-residue fragments,all fragments are applied in the process of fragment assembly.

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SNUGDOCK

Category: 
Docking

Hello,

I am trying to run SnugDock locally, and I follow the well explained demo. I noticed that makefab.pl script is missing. It should be in the demo / rosettascripts directory but its not there. Also, there is a link in the README.md file but its blocked by a usename and password....

Can anyone help?

 

Thanks,

Adva

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